A new high-performance liquid chromatographic method for the quality control of bioconverted Mori Folium extracts with appropriate marker compounds related to antidiabetes

Abstract A HPLC method was developed to assess the enhanced bioactivity of bioconverted Mori Folium (MF) extract and apply a quality-control system. Liquid-liquid extraction applied observe changes in chemical composition through enzymatic bioconversion. The ethyl acetate layer used for quality control anti-diabetic functional MF product. high-performance liquid chromatography analyze five marker compounds (syringaldehyde, trans-caffeic acid, morin 3-O-β-D-glucopyranoside, astragalin, moracin M 3′-O-β-glucopyranoside) within 60 min. Optimized sample preparation sonication 44 min water-to-material ratio 102.5 mL/g by response surface methodology. Comparing water extract, acid syringaldehyde contents were increased 1.89 times 2.70 at Viscozyme-bioconverted material applying method. This can be based on suitable bioactive markers also develop foods containing Folium.